Microscopic Agglutination Test Mat

Microscopic agglutination test mat bertujuan mendeteksi ada tidaknya infeksi leptospirosis dalam tubuh pasien.

Microscopic agglutination test mat. The microscopic agglutination test mat is the reference test method for the serodiagnosis of leptospirosis both in humans and in animals world health organization who 2003. Microscopic agglutination test mat for leptospirosis diagnosis abstract leptospirosis is a disease infecting human that has varying symptoms which makes it difficult to differentiate to other diseases. The microscopic agglutination test mat is the gold standard for sero diagnosis of leptospirosis because of its unsurpassed diagnostic specificity. The centers for disease control and prevention cdc laboratory in atlanta georgia.

1973 is the most widely used serological test for leptospirosis thiermann and garrett 1983. Igm elisa serion virion and immunochromatography test leptocheck wb are two immunodiagnostic assays for leptospirosis. 1965 and modified by cole et al. The centers for disease control and prevention cdc laboratory in atlanta georgia performs the mat using 23 leptospire antigens.

Microscopic agglutination test mat the mat which was originally described by galton et al. In 1926 schuffner and mochtar described the phenomenon of agglutination and lysis with human and animal sera and made their dilution series with drops from one and the same pipette. Hardjo infection in groups or herds of cattle. It uses panels of live leptospires ideally recent isolates representing the circulating serovars from the area where the patient became infected.

A 4 fold rise in mat titer between acute and convalescent sera with any of these antigens confirms the. Leptospirosis is diagnosed on clinical grounds and confirmed by microscopic agglutination test mat. World organisation for animal health oie 2008. Antileptospire antibodies in these samples are detected using the microscopic agglutination test mat.

Leptospirosis in taiwan 2001 2006. Their sensitivity specificity and applicability in sri lanka have not been systematically evaluated. Penyakit ini ditularkan melalui urine binatang seperti anjing tikus dan hewan ternak. The microscopic agglutination test mat is the basis of serological diagnosis and classification.

Laboratory confirmed diagnosis of leptospirosis was defined as a 4 fold rise in titer between paired samples or a single titer of. This test detects antibodies to specific serovars using live leptospiral antigens and can be performed on serum. Antileptospire antibodies in these samples are detected using the microscopic agglutination test mat. Currently diagnosing this disease is performed by serological examination using microscopic agglutination test.

The reaction was read after 16 20 hours.