Microscopic Agglutination Test Mat Leptospirosis

World organisation for animal health oie 2008.
Microscopic agglutination test mat leptospirosis. It uses panels of live leptospires ideally recent isolates representing the circulating serovars from the area where the patient became infected. 1973 is the most widely used serological test for leptospirosis thiermann and garrett 1983. Microscopic agglutination test mat for the diagnosis of leptospirosis and serotyping of leptospires h. The mixture is then examined under a dark field microscope to look for agglutination.
Hardjo infection in groups or herds of cattle. The mat is best used as a screening test when investigating the possibility of l. 1965 and modified by cole et al. The microscopic agglutination test mat is the basis of serological diagnosis and classification.
Penyakit ini ditularkan melalui urine binatang seperti anjing tikus dan hewan ternak. The microscopic agglutination test mat is the gold standard for sero diagnosis of leptospirosis because of its unsurpassed diagnostic specificity. Jun 13 2018 author. What is the role of microscopic agglutination test mat in the diagnosis of leptospirosis.
The microscopic agglutination test mat is the reference test method for the serodiagnosis of leptospirosis both in humans and in animals world health organization who 2003. Mat is a test where serial dilutions of patient sera are mixed with different serovars of leptospira. This test detects antibodies to specific serovars using live leptospiral antigens and can be performed on serum. Microscopic agglutination test mat mat is regarded as a gold standard method for the diagnosis of leptospirosis but it may be only available in reference laboratories.
Microscopic agglutination test mat bertujuan mendeteksi ada tidaknya infeksi leptospirosis dalam tubuh pasien. Michael stuart bronze md. The microscopic agglutination test mat is the reference test for the diagnosis of leptospirosis. Microscopic agglutination test mat the mat which was originally described by galton et al.
Seroconversion with a 4 fold or larger rise in titer between acute and convalescent phase serum specimens. Leptospirosis dapat ditularkan melalui tanah atau perairan yang terkontaminasi oleh urine. Sandra g gompf md facp fidsa. The highest dilution where 50 agglutination occurs is the.
Microscopic agglutination test mat confirmatory serologic testing available at cdc acute and convalescent serum samples collected 7 14 days apart is ideal. Kenapa microscopic agglutination test diperlukan. A commercially available slide agglutination test sat for the diagnosis of human leptospirosis was evaluated by comparing it to an immunoglobulin m igm enzyme linked immunosorbent assay elisa and to the microscopic agglutination test mat. For all 108 patients leptospirosis was diagnosed on the basis of a fourfold or greater increase in titer by mat seroconversion and all but 1 of.