Mat Testing Leptospirosis

Wild mammals typically rodents are the primary natural reservoir for pathogenic strains of leptospira however domestic animals eg dogs also represent a major source of human infection.

Mat testing leptospirosis. Korver department of biomedical research royal tropical institute meibergdreef 39 11005 az amsterdam the netherlands get pdf file. If the level of antibodies called a titer is high enough or can be shown to be rising over time then infection is confirmed. Leptospirosis is a zoonotic disease of worldwide prevalence though the majority of infections occur in warm tropical climates. Multilocus sequence typing mlst will be performed on clinical samples that have tested pcr positive.

What is the mat test for leptospirosis. Testing of 2 serum samples collected 7 to 14 days apart and representing acute and convalescent phase titers is recommended. World organisation for animal health oie 2008. 3 4 6 leptospiral infections usually are confirmed by a 4 fold increase in mat titers between acute and convalescent phase sera which is considered the.

To provide our companion animal and zoo animal clients a more complete diagnostic package we have combined the traditional leptospira serology assay mat with pcr. The mat is an excellent test but it. Testing with one sample assay for leptospirosis is not ideal and a combination of serology and organism detection is recommended. The majority of leptospirosis cases are identified through mat serology.

Curr opin infect dis. Therefore the diagnosis of leptospirosis in dogs mainly is based on mat for serological detection of leptospira specific antibodies and pcr for detection of their nucleic acids. The microscopic agglutination test mat is the basis of serological diagnosis and classification. Leptospirosis in taiwan an underestimated.

18 a 4 fold increase in titer indicates seroconversion and a diagnosis of leptospirosis. The mat or microscopic agglutination test detects the presence of antibodies against leptospira in a dog s blood. Laboratory testing is a critical component to aiding in the clinical diagnosis of this challenging disease. Microscopic agglutination test mat for the diagnosis of leptospirosis and serotyping of leptospires h.

19 20 a single acute. Leptospira are gram negative spirochetes with at least 20. Pathogenesis immunity and diagnosis. The microscopic agglutination test mat is the reference test method for the serodiagnosis of leptospirosis both in humans and in animals world health organization who 2003.

Does the mat always work.