Mat Leptospirosis Diagnosis

Mat is regarded as a gold standard method for the diagnosis of leptospirosis but it may be only available in reference laboratories.

Mat leptospirosis diagnosis. The laboratory diagnosis of leptospirosis is challenging. Leptospirosis also known as weil s disease grippotyphosa and canicola is a disease caused by bacteria leptospira interrogans that produce a wide range of symptoms that may occur in two phases. 2 4 for this test the patient s serum is sent to a referral laboratory where it is combined with live leptospires. Mat has high sensitivity and detects serovar specific antibodies.

Some patients may develop kidney or liver failure respiratory failure meningitis or even death the bacterial infection is spread by the urine of infected animals from many species both. The mat is the gold standard when testing for leptospirosis. One can start to detect antibodies to leptospira 7 10 days after the onset of the disease. In leptospirosis antibodies can be demonstrated in the serum by mat.

Mat is considered the gold standard test for diagnosis of leptospirosis. The microscopic agglutination test mat is the gold standard for sero diagnosis of leptospirosis because of its unsurpassed diagnostic specificity. Association of level of leptospiremia and clinical manifestations in sri lanka. Utility of quantitative polymerase chain reaction in leptospirosis diagnosis.

Mat has high sensitivity and detects serovar specific antibodies. The only sensitive and specific test accurate at the acute phase of the disease is polymerase chain reaction pcr which is not available in most high endemic areas and the serological reference method by micro agglutination testing mat is restricted to reference laboratories. During the acute phase of the disease mat is not specific in detecting a serotype of leptospira because of cross reactivity between the serovars. Mat titres of 1 100 to 1 800 are diagnostic of leptospirosis.

Clin infect dis. Detection of antibodies using the microscopic agglutination test mat has been the most common diagnostic method used for the diagnosis of canine leptospirosis 10 vaccination with commercially available leptospirosis vaccines will produce detectable mat titers 10 see algorithm 2 for more information on mat results. A fourfold or greater rise in titre of two sera taken at symptoms onset and three to 10 days of disease onset confirms the diagnosis. It has unsurpassed specificity but its sensitivity is low compared to elisa igm slide agglutination test sat.